1. Introduction; Part I. The Basis of Genetic Engineering: 2. Introducing molecular biology; 3. Working with nucleic acids; 4. The tools of the trade; Part II. The Methodology of Gene Manipulation: 5. Host cells and vectors; 6. Cloning strategies; 7. The polymerase chain reaction; 8. Selection, screening and analysis of recombinants; 9. Bioinformatics; Part III. Genetic Engineering in Action: 10. Understanding genes, genomes and 'otheromes'; 11. Genetic engineering and biotechnology; 12. Medical and forensic applications of gene manipulation; 13. Transgenic plants and animals; 14. The other sort of cloning; 15. Brave new world or genetic nightmare?